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He study period (1 h) was used to measure alveolar fluid movement.
The lungs were removed, andPage 3 of(page number not for citation purposes)Respiratory Research 2005, 6:http://respiratory-research.com/content/6/1/Table 1: Analysis of the bronchoalveolar lavage All the animals who received I-BRD9 Autophagy sterile beads were included in the sterile group and compared to the control and pneumonic groups at respectively 2, 5, 8, 15 and 21 days post instillation.- 7 Sterile groups (St) received an intratracheal instillation of sterile beads and were studied at different days after inoculation: St 1, St 2, St 5, St 8, St PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/28110187 15, St 21 and St 28. - 7 Pneumonic groups (Pn) received an intratracheal instillation of Pseudomonas containing beads and were studied at different days after inoculation: Pn 1, Pn 2, Pn 5, Pn 8, Pn 15, Pn 21, Pn 28.Statistical analysis Comparisons between two groups were made using an unpaired, two tailed Student‘s t-test. Comparisons between more than two groups were made using a one way analysis of variance with post hoc test for multiple comparisons. A value of p < 0.05 was considered as significant. The data are expressed as means ?SD.Total cells (?106)/mL Ctr St Pn 2 Pn 5 Pn 8 Pn 15 Pn 21 0.4 ?0.1 3.2 ?0.6 10.5 ?2.9* 7.9 ?1.7* 4.9 ?1.0 4.0 ?0.8 4.9 ?1.PMNs ( ) 0.5 ?0.4 5.6 ?4.4 79.8 ?5.2* 19.0 ?8.0 3.8 ?0.7 1.2 ?0.6 2.0 ?0.Macrophages ( ) 98.5 ?0.5 92.9 ?4.4 19.0 ?4.6* 79.3 ?8.4 95.5 ?1.0 98.8 ?0.6 97.2 ?0.Footnote: Data are mean (?SD). Comparisons between groups were made using analysis of variance with post hoc for multiple comparisons. *p < 0.05 vs the other groups. PMNs: Polymorphonuclear neutrophils, Ctr: Control group, St: Sterile group, Pn: Pneumonic groupResultsPseudomonas beads instillation is associated with the development of a chronic infection Clinically, a major weight loss was observed from the second day in P. aeruginosa beads infected animals compared to the sterile beads groups (Figure 1). 5 of the infected animals died within the first 48 hours after inoculation, none did in the sterile groups.Weight (g) 450 400 350 300 250 200 D1 D2 D5 D8 days D15 D21 DSt Pn* * *Prior to the instillation, the size of the inoculum was 7.9 105 ?1.5 105 CFU/mL. Lung bacterial load reached a peak on the second day of the infection; from the 5th day, a progressive decrease occurred to finally remain steady between the 15th day (8.25 ?5.2 104 CFU/mL) and the 3rd week (1.67 ?1.63 105 CFU/mL). Total broncho-alveolar lavage (BAL) cells slightly increased in the sterile beads group, the difference was however not statistically significant compared to the control group, the analysis showed that the number of cells peaked on the second day and was constituted, at that time, of 25 polymorphonuclear cells and 75 macrophages. The results were not statistically different over time and therefore pooled in Table 1. In the infected groups, alveolar cellularity was maximum on the 2nd day mostly polymorphonuclear‘s neutrophils (PMN). From the 8th day, the relative number of PMN progressively decreased as alveolar macrophages increased.
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